Product Name:
Mouse Apolipoprotein A1 (apo-A1) ELISA Kit
Cat.#:
JL34707
Regulatory Status:
Alternative:
Apo-A1; ApoA-1 Milano; ProapoA-I; Proapolipoprotein A-I; Truncated apolipoprotein A-I
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
15.62-1000ng/mL
Sensitivity:
5.39ng/mL
Specificity:
This kit recognizes Mouse apo-A1 in samples. No significant cross-reactivity or interference between Mouse apo-A1 and analogues was observed.
Sample Type:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Procedure:

Research Area:
Metabolism
Product Datasheets
| apo-A1
Apolipoprotein AI (Apo-AI) is a protein that is encoded by the APOA1 gene. As the major component of HDL particles, it has a specific role in lipid metabolism.Chylomicrons secreted from the intestinal enterocyte also contain Apo-AI, but it is quickly transferred to HDL in the bloodstream.The protein, as a component of HDL particles, enables efflux of fat molecules by accepting fats from within cells (including macrophages within the walls of arteries which have become overloaded with ingested fats from oxidized LDL particles) for transport (in the water outside cells) elsewhere, including back to LDL particles or to the liver for excretion.| Typical data
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.Concentration (ng/mL) | 1000 | 500 | 250 | 125 | 62.5 | 31.25 | 15.62 | 0 |
OD | 2.52 | 1.75 | 1.08 | 0.71 | 0.46 | 0.32 | 0.23 | 0.09 |
Corrected OD | 2.43 | 1.66 | 0.99 | 0.62 | 0.37 | 0.23 | 0.14 | - |
| Repeatability
Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%| Recovery
The recovery of Mouse apo-A1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.Sample Type | Range(%) | Average Recovery(%) |
Serum(n=8) | 84-101 | 96 |
Plasma(n=8) | 92-105 | 102 |
Cell Culture Supernates(n=8) | 97-108 | 105 |
| Linearity
Samples were spiked with high concentrations of Mouse apo-A1 and diluted with Universal Diluent to produce samples with values within the range of the assay.Serum(n=4) | Plasma(n=4) | Cell Culture Supernates(n=4) | ||
1 : 2 | Range (%) | 84-95 | 88-96 | 90-110 |
Average (%) | 91 | 93 | 96 | |
1 : 4 | Range (%) | 89-103 | 87-108 | 105-115 |
Average (%) | 94 | 98 | 108 |






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Quantity
Price ($)
48T


420 / 420
96T


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